MyBioSource has expanded its research reagent catalog with the addition of a validated sheep anti-rat calretinin polyclonal antibody (MBS502148), designed to support advanced neuroscience and cellular calcium signaling studies. The antibody specifically targets calretinin (CALB2), a 29kDa calcium-binding protein predominantly expressed in neurons throughout the central nervous system, retina, and other tissues. This development holds significant importance for neuroscience research focused on neurodegeneration, calcium signaling mechanisms, cellular calcium buffering, and auditory neuron studies.
The anti-CALB2 antibody demonstrates high specificity by recognizing the rat calretinin protein at approximately 29 kDa, as confirmed through Western blot analysis of rat brain lysate. Due to 100% sequence homology, researchers can expect potential cross-reactivity with mouse, human, and non-human primate calretinin, making it valuable for comparative studies across species. The antibody was developed using purified guinea pig calretinin protein as the immunogen. Researchers can utilize this reagent in multiple validated techniques including Western Blot (WB), Immunohistochemistry (IHC) on formalin or paraformaldehyde perfused rat brain tissues, and Immunofluorescence (IF).
The antibody undergoes Protein A purification to ensure high specificity and minimal background noise, and it remains stable when stored at -20°C with precautions against repeated freeze/thaw cycles. Calretinin serves crucial roles in intracellular calcium buffering, cellular homeostasis, and neuroprotection, making it an essential marker for investigating calcium dynamics and neuronal resistance to excitotoxicity. The antibody supports research in academic settings aimed at elucidating calcium regulation mechanisms, neuronal function, and related biological pathways while contributing to literature in neurodegeneration, auditory neuroscience, and developmental biology.
Researchers can access this product through www.mybiosource.com. The anti-CALB2 antibody is intended for research purposes only and is not suitable for diagnostic, therapeutic, or clinical applications. Scientists should adhere to appropriate laboratory safety guidelines and validate antibody performance under their specific experimental conditions.
